Vitamin B1 and cocarboxylase in animal tissues1

Vitamin B1

Vitamin B1 is a watersoluble vitamin. It consists out of a pyrimidineand a thiazol ring, which are synthesized independently and linked afterwards.Vitamin B1 is de novo produced by many plants and microorganisms. Humans and animals have to take it up by nutrition. They are only able to do the phosphorylation reaction of the thiamin molecule. Thiamin is found free and as mono-, diand triphosphat...

Intestinal Flora and Vitamin B1

Vitamin-B1 Deficiency

Thiamine and Cocarboxylase Concentration

In patients with congestive heart failure, thiamine deficiency has been demonstrated by the test-dose method (1, 2), by measurement of the lactate-pyruvate ratio of the blood (3), and by determination of blood cocarboxylase (4). The oxidation of pyruvate to acetate requires thiamine in the form of cocarboxylase (thiamine pyrophosphate). Olson, Pearson, Miller, and Stare have shown that thiamine...

Vitamin B1 Deficient Animals and Poliomyelitis *

In previous experiments,2 an unsuccessful attempt was made to acclimate monkey cord virus to white rats, which had been made completely vitamin B1 deficient by feeding them a vitamin B1 free diet for 30 days prior to the start of the experiment. It was thought that our failure might have been due to the fact that we did not have the animals in a proper state or stage of deficiency; perhaps the ...